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fncas12a  (Addgene inc)


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    Addgene inc fncas12a
    a PAM compatibilities of the Cas12a variants in human cells. A heat map showing the average indel frequencies induced by the Cas12a variants at the 15 PAMs that are currently known to be recognized by Cas12a. b Pearson correlation coefficients between the Cas12a variant induced indel frequencies measured at the target sequences containing the same protospacers 7 days after transduction of Cas12a variant lentivirus into libraries integrated HEK293T cells. c Scatter plots showing the correlations of the five cases that are labeled as 1, 2, 3, 4 and 5 in the b . The Spearman correlation coefficient ( ρ ) and the Pearson correlation coefficient ( r ) are shown. In b and c , 15 PAM sequences were used for the analyses. d Comparison of the frequency of indels induced by the Cas12a variants at TTTA, TTTC, TTTG PAM; The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences ( n ) are as follows: n = 1195, 1223, 1224, 1157, 1191, 1178, 1171 for TTTA, n = 1198, 1642, 1621, 1560, 1591, 1585, 1569 for TTTC, n = 1711, 1753, 1729, 1682, 1706, 1694, 1687 for TTTG (As variants); n = 1240, 1232 for TTTA, n = 1649, 1645, n = 1762, 1775 for TTTG (Eb variants); n = 1295, 1283 for TTTA, n = 1675, 1674, n = 1809, 1805 for TTTG (Lb2 variants); n = 1250 for TTTA, n = 1653 for TTTC, n = 1780 for TTTG (CeCas12a); n = 1202, 1216, 1244, 1300, 1243, 1199, 1210, 1165, 1174 for TTTA, n = 1629, 1623, 1682, 1615, 1646, 1647, 1597, 1596, 1623 for TTTC, n = 1753, 1755, 1809, 1738, 1780, 1778, 1699, 1697, 1748 for TTTG (LbCas12a variants); n = 1205, 1224, 1201 for TTTA, n = 1622, 1632, 1618 for TTTC, n = 1742, 1761, 1745 for TTTG <t>(FnCas12a</t> variants); paired t -test, two tailed. Source data are provided as a Source Data file.
    Fncas12a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fncas12a/pY004+(pcDNA3%2E1-hFnCpf1)+(Plasmid+%2369976)/pmc11953374-163-6-7
    Average 92 stars, based on 24 article reviews
    fncas12a - by Bioz Stars, 2026-09
    92/100 stars

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    1) Product Images from "Highly parallel profiling of the activities and specificities of Cas12a variants in human cells"

    Article Title: Highly parallel profiling of the activities and specificities of Cas12a variants in human cells

    Journal: Nature Communications

    doi: 10.1038/s41467-025-57150-9

    a PAM compatibilities of the Cas12a variants in human cells. A heat map showing the average indel frequencies induced by the Cas12a variants at the 15 PAMs that are currently known to be recognized by Cas12a. b Pearson correlation coefficients between the Cas12a variant induced indel frequencies measured at the target sequences containing the same protospacers 7 days after transduction of Cas12a variant lentivirus into libraries integrated HEK293T cells. c Scatter plots showing the correlations of the five cases that are labeled as 1, 2, 3, 4 and 5 in the b . The Spearman correlation coefficient ( ρ ) and the Pearson correlation coefficient ( r ) are shown. In b and c , 15 PAM sequences were used for the analyses. d Comparison of the frequency of indels induced by the Cas12a variants at TTTA, TTTC, TTTG PAM; The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences ( n ) are as follows: n = 1195, 1223, 1224, 1157, 1191, 1178, 1171 for TTTA, n = 1198, 1642, 1621, 1560, 1591, 1585, 1569 for TTTC, n = 1711, 1753, 1729, 1682, 1706, 1694, 1687 for TTTG (As variants); n = 1240, 1232 for TTTA, n = 1649, 1645, n = 1762, 1775 for TTTG (Eb variants); n = 1295, 1283 for TTTA, n = 1675, 1674, n = 1809, 1805 for TTTG (Lb2 variants); n = 1250 for TTTA, n = 1653 for TTTC, n = 1780 for TTTG (CeCas12a); n = 1202, 1216, 1244, 1300, 1243, 1199, 1210, 1165, 1174 for TTTA, n = 1629, 1623, 1682, 1615, 1646, 1647, 1597, 1596, 1623 for TTTC, n = 1753, 1755, 1809, 1738, 1780, 1778, 1699, 1697, 1748 for TTTG (LbCas12a variants); n = 1205, 1224, 1201 for TTTA, n = 1622, 1632, 1618 for TTTC, n = 1742, 1761, 1745 for TTTG (FnCas12a variants); paired t -test, two tailed. Source data are provided as a Source Data file.
    Figure Legend Snippet: a PAM compatibilities of the Cas12a variants in human cells. A heat map showing the average indel frequencies induced by the Cas12a variants at the 15 PAMs that are currently known to be recognized by Cas12a. b Pearson correlation coefficients between the Cas12a variant induced indel frequencies measured at the target sequences containing the same protospacers 7 days after transduction of Cas12a variant lentivirus into libraries integrated HEK293T cells. c Scatter plots showing the correlations of the five cases that are labeled as 1, 2, 3, 4 and 5 in the b . The Spearman correlation coefficient ( ρ ) and the Pearson correlation coefficient ( r ) are shown. In b and c , 15 PAM sequences were used for the analyses. d Comparison of the frequency of indels induced by the Cas12a variants at TTTA, TTTC, TTTG PAM; The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences ( n ) are as follows: n = 1195, 1223, 1224, 1157, 1191, 1178, 1171 for TTTA, n = 1198, 1642, 1621, 1560, 1591, 1585, 1569 for TTTC, n = 1711, 1753, 1729, 1682, 1706, 1694, 1687 for TTTG (As variants); n = 1240, 1232 for TTTA, n = 1649, 1645, n = 1762, 1775 for TTTG (Eb variants); n = 1295, 1283 for TTTA, n = 1675, 1674, n = 1809, 1805 for TTTG (Lb2 variants); n = 1250 for TTTA, n = 1653 for TTTC, n = 1780 for TTTG (CeCas12a); n = 1202, 1216, 1244, 1300, 1243, 1199, 1210, 1165, 1174 for TTTA, n = 1629, 1623, 1682, 1615, 1646, 1647, 1597, 1596, 1623 for TTTC, n = 1753, 1755, 1809, 1738, 1780, 1778, 1699, 1697, 1748 for TTTG (LbCas12a variants); n = 1205, 1224, 1201 for TTTA, n = 1622, 1632, 1618 for TTTC, n = 1742, 1761, 1745 for TTTG (FnCas12a variants); paired t -test, two tailed. Source data are provided as a Source Data file.

    Techniques Used: Variant Assay, Transduction, Labeling, Comparison, Two Tailed Test

    Related Articles

    Plasmid Preparation:

    Article Title: Two novel mgCas12a proteins from the human gut metagenome exhibit reduced collateral DNase activity
    Article Snippet: .. Plasmid construction for protein purification The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: RPA assay coupled with CRISPR/Cas12a system for the detection of seven Eimeria species in chicken fecal samples.
    Article Snippet: Chicken coccidiosis is one of the most common and economically important diseases in the global poultry industry, and it is caused by at least one of the seven Eimeria species.. A simple and reliable way to distinguish Eimeria species in infected chicken is critical for the surveillance, control, and eradication of chicken coccidiosis.. In this study, a recombinase polymerase amplification (RPA) assay coupled with the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system (RPA–CRISPR/Cas12a) was developed for the detection of Eimeria species in chicken fecal samples.

    Article Title: Highly parallel profiling of the activities and specificities of Cas12a variants in human cells
    Article Snippet: .. AsCas12a (Addgene, #69982), LbCas12a (Addgene, #69988), FnCas12a (Addgene, #69976), Lb2Cas12a (Addgene, #69983) human expression plasmids were purchased from the non-profit plasmid repository Addgene. .. To prepare the lentiviral vectors, we removed the Cas9-encoding sequence from the lentiCas9-Blast plasmid (Addgene, #52962), and cloned Cas12a-encoding sequences between XbaI and BamHI.

    Protein Purification:

    Article Title: Two novel mgCas12a proteins from the human gut metagenome exhibit reduced collateral DNase activity
    Article Snippet: .. Plasmid construction for protein purification The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Synthesized:

    Article Title: Two novel mgCas12a proteins from the human gut metagenome exhibit reduced collateral DNase activity
    Article Snippet: .. Plasmid construction for protein purification The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome.
    Article Snippet: .. The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome
    Article Snippet: .. The coding sequences of mgCas12a-1 , mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Clone Assay:

    Article Title: Two novel mgCas12a proteins from the human gut metagenome exhibit reduced collateral DNase activity
    Article Snippet: .. Plasmid construction for protein purification The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a.
    Article Snippet: .. Plasmids harboring full-length LbCas12a and FnCas12a were obtained from Addgene (catalog numbers 113431 and 113432, respectively) and cloned into pET19-based expression vectors (pIF162 and pIF1042 for LbCas12a and FnCas12a, respectively). ..

    Article Title: Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome.
    Article Snippet: .. The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome
    Article Snippet: .. The coding sequences of mgCas12a-1 , mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: The heat is on: a simple method to increase genome editing efficiency in plants
    Article Snippet: .. AsCas12a, FnCas12a, and LbCas12a were amplified from pY010, pY004, and pY016, respectively and cloned into pGGC000 using restriction ligation. pY010, pY004, and pY016 were gifts from Feng Zhang (Addgene plasmids # 69,982, # 69,976, and # 69,988; [ ]). ..

    Sequencing:

    Article Title: Two novel mgCas12a proteins from the human gut metagenome exhibit reduced collateral DNase activity
    Article Snippet: .. Plasmid construction for protein purification The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: RPA assay coupled with CRISPR/Cas12a system for the detection of seven Eimeria species in chicken fecal samples.
    Article Snippet: Chicken coccidiosis is one of the most common and economically important diseases in the global poultry industry, and it is caused by at least one of the seven Eimeria species.. A simple and reliable way to distinguish Eimeria species in infected chicken is critical for the surveillance, control, and eradication of chicken coccidiosis.. In this study, a recombinase polymerase amplification (RPA) assay coupled with the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system (RPA–CRISPR/Cas12a) was developed for the detection of Eimeria species in chicken fecal samples.

    Article Title: A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing
    Article Snippet: For pAAV-CMV-Cas-puro, fragments of SpCas9 , AsCas12a , and FnCas12a were PCR-amplified using pSpCas9(BB)-2A-puro (PX459) V2.0 (Addgene no. 62988), pCMV-T7-AsCas12a-P2A-EGFP (Addgene no. 160140), and FnoCas12a-2C-NLS (Nuclear localization sequence) in pCSDest (Addgene no. 126639) as templates. pAAV backbone was PCR-amplified with primers pAAV-F and pAAV-R using pAAV-CMV-SauriCas9-puro (Addgene no. 135965) as a template. .. For pAAV-CMV-Cas-puro, fragments of SpCas9 , AsCas12a , and FnCas12a were PCR-amplified using pSpCas9(BB)-2A-puro (PX459) V2.0 (Addgene no. 62988), pCMV-T7-AsCas12a-P2A-EGFP (Addgene no. 160140), and FnoCas12a-2C-NLS (Nuclear localization sequence) in pCSDest (Addgene no. 126639) as templates. pAAV backbone was PCR-amplified with primers pAAV-F and pAAV-R using pAAV-CMV-SauriCas9-puro (Addgene no. 135965) as a template. .. These fragments of SpCas9 , AsCas12a , and FnCas12a were recombined with pAAV backbone using the NEBuilder HiFi DNA Assembly Cloning Kit [New England Biolabs (NEB), E5520] to generate pAAV-CMV-Cas-puro vectors.

    Article Title: Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome.
    Article Snippet: .. The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome
    Article Snippet: .. The coding sequences of mgCas12a-1 , mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Modification:

    Article Title: Two novel mgCas12a proteins from the human gut metagenome exhibit reduced collateral DNase activity
    Article Snippet: .. Plasmid construction for protein purification The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome.
    Article Snippet: .. The coding sequences of mgCas12a-1, mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Article Title: Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome
    Article Snippet: .. The coding sequences of mgCas12a-1 , mgCas12a-2 (synthesized by Bionics, Seoul, South Korea), AsCas12a (Addgene #102565), FnCas12a (Addgene #102565), and LbCas12a (Addgene #102566) were cloned in-frame between the sequence encoding a polyhistidine tag and a bipartite nuclear localization signal (BPNLS) in a modified pET28a vector. ..

    Expressing:

    Article Title: Highly parallel profiling of the activities and specificities of Cas12a variants in human cells
    Article Snippet: .. AsCas12a (Addgene, #69982), LbCas12a (Addgene, #69988), FnCas12a (Addgene, #69976), Lb2Cas12a (Addgene, #69983) human expression plasmids were purchased from the non-profit plasmid repository Addgene. .. To prepare the lentiviral vectors, we removed the Cas9-encoding sequence from the lentiCas9-Blast plasmid (Addgene, #52962), and cloned Cas12a-encoding sequences between XbaI and BamHI.

    Article Title: Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a.
    Article Snippet: .. Plasmids harboring full-length LbCas12a and FnCas12a were obtained from Addgene (catalog numbers 113431 and 113432, respectively) and cloned into pET19-based expression vectors (pIF162 and pIF1042 for LbCas12a and FnCas12a, respectively). ..

    Polymerase Chain Reaction:

    Article Title: A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing
    Article Snippet: For pAAV-CMV-Cas-puro, fragments of SpCas9 , AsCas12a , and FnCas12a were PCR-amplified using pSpCas9(BB)-2A-puro (PX459) V2.0 (Addgene no. 62988), pCMV-T7-AsCas12a-P2A-EGFP (Addgene no. 160140), and FnoCas12a-2C-NLS (Nuclear localization sequence) in pCSDest (Addgene no. 126639) as templates. pAAV backbone was PCR-amplified with primers pAAV-F and pAAV-R using pAAV-CMV-SauriCas9-puro (Addgene no. 135965) as a template. .. For pAAV-CMV-Cas-puro, fragments of SpCas9 , AsCas12a , and FnCas12a were PCR-amplified using pSpCas9(BB)-2A-puro (PX459) V2.0 (Addgene no. 62988), pCMV-T7-AsCas12a-P2A-EGFP (Addgene no. 160140), and FnoCas12a-2C-NLS (Nuclear localization sequence) in pCSDest (Addgene no. 126639) as templates. pAAV backbone was PCR-amplified with primers pAAV-F and pAAV-R using pAAV-CMV-SauriCas9-puro (Addgene no. 135965) as a template. .. These fragments of SpCas9 , AsCas12a , and FnCas12a were recombined with pAAV backbone using the NEBuilder HiFi DNA Assembly Cloning Kit [New England Biolabs (NEB), E5520] to generate pAAV-CMV-Cas-puro vectors.

    Amplification:

    Article Title: The heat is on: a simple method to increase genome editing efficiency in plants
    Article Snippet: .. AsCas12a, FnCas12a, and LbCas12a were amplified from pY010, pY004, and pY016, respectively and cloned into pGGC000 using restriction ligation. pY010, pY004, and pY016 were gifts from Feng Zhang (Addgene plasmids # 69,982, # 69,976, and # 69,988; [ ]). ..

    Ligation:

    Article Title: The heat is on: a simple method to increase genome editing efficiency in plants
    Article Snippet: .. AsCas12a, FnCas12a, and LbCas12a were amplified from pY010, pY004, and pY016, respectively and cloned into pGGC000 using restriction ligation. pY010, pY004, and pY016 were gifts from Feng Zhang (Addgene plasmids # 69,982, # 69,976, and # 69,988; [ ]). ..



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    a PAM compatibilities of the Cas12a variants in human cells. A heat map showing the average indel frequencies induced by the Cas12a variants at the 15 PAMs that are currently known to be recognized by Cas12a. b Pearson correlation coefficients between the Cas12a variant induced indel frequencies measured at the target sequences containing the same protospacers 7 days after transduction of Cas12a variant lentivirus into libraries integrated HEK293T cells. c Scatter plots showing the correlations of the five cases that are labeled as 1, 2, 3, 4 and 5 in the b . The Spearman correlation coefficient ( ρ ) and the Pearson correlation coefficient ( r ) are shown. In b and c , 15 PAM sequences were used for the analyses. d Comparison of the frequency of indels induced by the Cas12a variants at TTTA, TTTC, TTTG PAM; The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences ( n ) are as follows: n = 1195, 1223, 1224, 1157, 1191, 1178, 1171 for TTTA, n = 1198, 1642, 1621, 1560, 1591, 1585, 1569 for TTTC, n = 1711, 1753, 1729, 1682, 1706, 1694, 1687 for TTTG (As variants); n = 1240, 1232 for TTTA, n = 1649, 1645, n = 1762, 1775 for TTTG (Eb variants); n = 1295, 1283 for TTTA, n = 1675, 1674, n = 1809, 1805 for TTTG (Lb2 variants); n = 1250 for TTTA, n = 1653 for TTTC, n = 1780 for TTTG (CeCas12a); n = 1202, 1216, 1244, 1300, 1243, 1199, 1210, 1165, 1174 for TTTA, n = 1629, 1623, 1682, 1615, 1646, 1647, 1597, 1596, 1623 for TTTC, n = 1753, 1755, 1809, 1738, 1780, 1778, 1699, 1697, 1748 for TTTG (LbCas12a variants); n = 1205, 1224, 1201 for TTTA, n = 1622, 1632, 1618 for TTTC, n = 1742, 1761, 1745 for TTTG <t>(FnCas12a</t> variants); paired t -test, two tailed. Source data are provided as a Source Data file.
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    a PAM compatibilities of the Cas12a variants in human cells. A heat map showing the average indel frequencies induced by the Cas12a variants at the 15 PAMs that are currently known to be recognized by Cas12a. b Pearson correlation coefficients between the Cas12a variant induced indel frequencies measured at the target sequences containing the same protospacers 7 days after transduction of Cas12a variant lentivirus into libraries integrated HEK293T cells. c Scatter plots showing the correlations of the five cases that are labeled as 1, 2, 3, 4 and 5 in the b . The Spearman correlation coefficient ( ρ ) and the Pearson correlation coefficient ( r ) are shown. In b and c , 15 PAM sequences were used for the analyses. d Comparison of the frequency of indels induced by the Cas12a variants at TTTA, TTTC, TTTG PAM; The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences ( n ) are as follows: n = 1195, 1223, 1224, 1157, 1191, 1178, 1171 for TTTA, n = 1198, 1642, 1621, 1560, 1591, 1585, 1569 for TTTC, n = 1711, 1753, 1729, 1682, 1706, 1694, 1687 for TTTG (As variants); n = 1240, 1232 for TTTA, n = 1649, 1645, n = 1762, 1775 for TTTG (Eb variants); n = 1295, 1283 for TTTA, n = 1675, 1674, n = 1809, 1805 for TTTG (Lb2 variants); n = 1250 for TTTA, n = 1653 for TTTC, n = 1780 for TTTG (CeCas12a); n = 1202, 1216, 1244, 1300, 1243, 1199, 1210, 1165, 1174 for TTTA, n = 1629, 1623, 1682, 1615, 1646, 1647, 1597, 1596, 1623 for TTTC, n = 1753, 1755, 1809, 1738, 1780, 1778, 1699, 1697, 1748 for TTTG (LbCas12a variants); n = 1205, 1224, 1201 for TTTA, n = 1622, 1632, 1618 for TTTC, n = 1742, 1761, 1745 for TTTG (FnCas12a variants); paired t -test, two tailed. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Highly parallel profiling of the activities and specificities of Cas12a variants in human cells

    doi: 10.1038/s41467-025-57150-9

    Figure Lengend Snippet: a PAM compatibilities of the Cas12a variants in human cells. A heat map showing the average indel frequencies induced by the Cas12a variants at the 15 PAMs that are currently known to be recognized by Cas12a. b Pearson correlation coefficients between the Cas12a variant induced indel frequencies measured at the target sequences containing the same protospacers 7 days after transduction of Cas12a variant lentivirus into libraries integrated HEK293T cells. c Scatter plots showing the correlations of the five cases that are labeled as 1, 2, 3, 4 and 5 in the b . The Spearman correlation coefficient ( ρ ) and the Pearson correlation coefficient ( r ) are shown. In b and c , 15 PAM sequences were used for the analyses. d Comparison of the frequency of indels induced by the Cas12a variants at TTTA, TTTC, TTTG PAM; The boxes represent the 25th, 50th, and 75th percentiles, and the whiskers represent the 10th and 90th percentiles. The numbers of analyzed target sequences ( n ) are as follows: n = 1195, 1223, 1224, 1157, 1191, 1178, 1171 for TTTA, n = 1198, 1642, 1621, 1560, 1591, 1585, 1569 for TTTC, n = 1711, 1753, 1729, 1682, 1706, 1694, 1687 for TTTG (As variants); n = 1240, 1232 for TTTA, n = 1649, 1645, n = 1762, 1775 for TTTG (Eb variants); n = 1295, 1283 for TTTA, n = 1675, 1674, n = 1809, 1805 for TTTG (Lb2 variants); n = 1250 for TTTA, n = 1653 for TTTC, n = 1780 for TTTG (CeCas12a); n = 1202, 1216, 1244, 1300, 1243, 1199, 1210, 1165, 1174 for TTTA, n = 1629, 1623, 1682, 1615, 1646, 1647, 1597, 1596, 1623 for TTTC, n = 1753, 1755, 1809, 1738, 1780, 1778, 1699, 1697, 1748 for TTTG (LbCas12a variants); n = 1205, 1224, 1201 for TTTA, n = 1622, 1632, 1618 for TTTC, n = 1742, 1761, 1745 for TTTG (FnCas12a variants); paired t -test, two tailed. Source data are provided as a Source Data file.

    Article Snippet: AsCas12a (Addgene, #69982), LbCas12a (Addgene, #69988), FnCas12a (Addgene, #69976), Lb2Cas12a (Addgene, #69983) human expression plasmids were purchased from the non-profit plasmid repository Addgene.

    Techniques: Variant Assay, Transduction, Labeling, Comparison, Two Tailed Test